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RBAM_004680

unknown
geneRBAM_004680
locusRBAM_004680
feature typeCDS
productABS72867.2 SH3 domain-containing protein
synonyms
homologF2 protein
start466949
stop467269
direction+
length354
essential
protein detected in LBnot detected
Genomic Context

Functional category containing this gene/protein

Category
Subcategory1
Subcategory2
Subcategory3

Phenotypes of a mutant

plant-bacteria interaction

Protein F2 (homolog of YP_001420098.1/RBAM_004680) possess an antifungal action and is efficient against Rhizoctonia cerealis.

Expression and Regulation

Operons
Transcripts
Primary TSS466873
Secondary TSS
Internal TSS
Antisense TSS

Sigma factors

Protein regulator

Additional regulation

RNA regulation_mode

This gene is a member of the following regulons

The protein


Catalyzed reaction/ biological activity
antifungal
E.C. number
Protein family
Domains
Effectors of protein activity
Structure
Malonylation
Acetylation
Localization
supernatant

Biological materials


Mutant
LacZ fusion
GFP fusion
FLAG-tag construct
Antibody
Additional information
The homolog protein (F2) was detected in supernatant of B. subtilis Z-14 and heterologous expressed in yeast Pichia pastoris. The recombinant protein rF2 was efficient against Fusarium oxysporum, Verticillium dahliae, Rhizoctonia cerealis. Resistant against heat, proteinase K and trypsin.

References


Zhang X, Guo X, Wu C, Li C, Zhang D, Zhu B

Isolation, heterologous expression and purification of a novel antifungal protein from Bacillus subtilis strain Z-14

Microb Cell Fact (2020) 19:214